Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 6;7(9):170146. doi: 10.1098/rsob.170146

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

PMC Copyright notice

Figure 1. — DnaE is required for both initiation and elongation of replication. Strains HVS597, HVS609p and HVS614p encode dnaC, polC or dnaE from the Pspac IPTG-inducible promoter. Strains were grown overnight at moderate inducer concentration and then were diluted and maintained in exponential phase (by serial dilutions) in the presence of the indicated IPTG concentrations (μM). Cell growth was monitored spectrophotometrically (a) and the ori/ter ratio was measured by qPCR (b). The ratio was determined just before the growth rate decrease/arrest (arrows in (a)). At these time points, cells suffer from a dramatic replication defect for about 2–3 generations. Bars represent mean values with standard errors of at least three independent cultures. (c) Exponentially growing wild-type cells (168) were treated with a lethal concentration of a HB-EMAU (10 µM). The ori/ter ratio was determined by qPCR at different time points upon HB-EMAU treatment. A representative experiment is shown.