Figure 5.

Mechanotyping of HL-60 cells treated with cytoskeleton-perturbing drugs using q-DC. HL-60 cells are treated with blebbistatin (Bleb), cytochalasin D (CytoD), and jasplakinolide (Jasp). (A) Density scatter plots show apparent elastic modulus, E_a, and fluidity, β, as functions of cell size, which is measured in the microfluidic channel before the cell enters the constriction. The cell diameter shown here appears to be larger than unconfined cells (Fig. S8A), as well as cells in the 10 μm height devices (Fig. 4), due to axial compression that occurs when the cell diameter is larger than the device height. Each dot represents a single cell. To compare data sets, we bin cells by size, as depicted by the dotted lines. Cell-size distributions are shown in Fig. S8. (B) Boxplots represent the size-binned distributions of E_a and β for cells with D_cell = 21 ± 1 μm, white lines represent the median, boxes represent the interquartile ranges, whiskers represent the 10th and 90th percentiles, and white squares represent the bootstrapped median. N > 500 for each cell type. Statistical significance is determined using the Mann-Whitney U test: ^∗∗p < 0.01, ^∗∗∗p < 0.001. To see this figure in color, go online.