Table 1.

Summary of identified Daphnia mtDNA mutations. Total depth represents the number of sequence coverage at a site. syn: synonymous substitution. non-syn: non-synonymous substitution.

position	sample	reference	mutation	total depth	mutation frequency	gene	effect	amino acid changes
839	57	C	T	28	0.357	ND2	syn	AAC(N) → AAU (N)
997	19	T	A	45	0.400	ND2	non-syn	UUA (L) → UAA (STOP)
1775	26	C	T	77	0.247	COI	syn	ACC(T) → ACU (T)
3594	37	C	A	140	0.164	COII	syn	UCC(S) → UCA (S)
3898	46	C	T	120	0.275	ATPase8	syn	UUC(F) → UUU (F)
5090	47	G	A	133	0.321	COIII	non-syn	UGG (W) → UGA (STOP)
7482	52	G	A	109	0.288	ND5	non-syn	GCU (A) → GUU (V)
8317	49	A	G	69	0.145	ND4	syn	ACU(T) → ACC (T)
8709	90	C	T	49	0.551	ND4	non-syn	GCA (A) → ACA (T)
8734	7	A	G	76	0.211	ND4	syn	CCU (P) → CCC (P)
9072	51	C	T	58	0.254	ND4	non-syn	GUG (V) → AUG (Start)
9141	33	T	C	66	0.182	ND4	non-syn	AUG (M) → GUG (V)
10 087	40	T	C	85	0.235	ND6	non-syn	UCC (S) → CCC (P)
10 217	96	T	C	76	0.171	ND6	non-syn	CUU (L) → CCU (P)
10 625	77	T	A	61	0.442	CytB	non-syn	UUA (L) → AUA (I)
11 775	60	T	C	121	0.147	ND1	non-syn	AGU (S) → GGU (G)
11 920	87	A	G	76	0.145	ND1	syn	UUU (F) → UUC (F)
12 801	55	T	C	88	0.159	rRNA-Large	non-coding	n.a.
13 533	87	C	T	94	0.191	rRNA-Large	non-coding	n.a.