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. 2017 Jul 17;2:20. doi: 10.1038/s41541-017-0021-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Detection of reversion mutants of the MP-12 vaccine during viral passages in MRC-5 cells. The MP-12 vaccine was passaged 25 times in MRC-5 cells at MOI of 0.01 or less at 3 days post infection. Virus RNA was purified from culture supernatants and analyzed for the presence of reversion mutations using Taqman probes (shown in Supplementary Fig. 1 and Supplementary Table 2). a M-segment nt. 795: HEX-795-C and FAM-795-T, b M-segment nt. 3564: HEX-3564-G and FAM-3564-A, c L-segment nt. 533: HEX-533-C and FAM-533-T, d L-segment nt. 3104: HEX-3104-A and FAM-3104-G, and e L-segment nt. 3750: HEX-3750-A and FAM-3750-G. Three independent studies are shown (Exp-1, 2, and 3). Relative percentages of RNA copy numbers for parental and reversion mutants are shown in blue and red bars, respectively. The bar labels in red represent the percentages of reversion mutants