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. 2017 Oct 4;7:12657. doi: 10.1038/s41598-017-12842-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Larvae infected under light exposure demonstrate greater recruitment of neutrophils and macrophages to the hindbrain ventricle. (a) Immunofluorescence detection of neutrophils at 1, 3, 6 and 9 hpi within the hindbrain region of infected Tg(lyz:DsRED2) ^nz50 larvae raised under light/dark, constant light, dark/light and constant dark conditions. (b) Immunofluorescence detection of macrophages at 1, 3, 6 and 9 hpi within the hindbrain region of infected Tg(mpeg1:Gal4-FF) ^gl25 ;Tg(UAS-E1b:nfsB.mCherry) ^c264 larvae raised under light/dark, constant light, dark/light and constant dark conditions. (c) Quantification of neutrophils in the hindbrain region of individual infected Tg(lyz:DsRED2) ^nz50 larvae, as detected in (a). (d) Quantification of macrophages in the hindbrain region of individual Tg(mpeg1:Gal4-FF) ^gl25 ;Tg(UAS-E1b:nfsB.mCherry) ^c264 larvae, as detected in (b). (e,f) Flow cytometry quantification of neutrophils and macrophages from Tg(lyz:DsRED2) ^nz50 and Tg(mpeg1:Gal4-FF) ^gl25;Tg(UAS-E1b:nfsB.mCherry) ^c264 larvae, respectively, raised under light/dark, constant light, dark/light and constant dark conditions. Data represent three biological replicates. Scale bar, 50 μm; data shown as mean ± s.d.; hpi, hours post-infection; p-values were calculated by one-way ANOVA with Tukey’s multiple comparisons test; ns, not significant.