Fig. 2.

Nano-11 is retained at the injection site and cleared with time. a Mice were injected in both calf muscles with 200 µg Alexa Fluor 647-labeled Nano-11 (1 and 2) or nonlabeled Nano-11 (control) with 10 µg OVA and imaged under a fluorescence imager at different time points. b Internal organs (from top to bottom: heart, lung, spleen, liver, kidneys, iliac lymph node, popliteal lymph nodes, and injection sites) of mice were harvested on day 21 and imaged. c Fluorescence intensity of the injection site over time expressed as percentage of the intensity at 0 h. Mean ± SEM of five mice (days 0, 1, 2, 7) or three mice (days 14 and 21). *Significantly different from day 2 fluorescence (p < 0.001)