Fig. 3.

Nano-11 induces transient inflammation at the injection site and is taken up by recruited cells. a Injection sites were collected at different time points after intramuscular injection of 200 µg Nano-11 combined with 10 µg ovalbumin. Cells were identified with monoclonal antibodies and phenol red (converted by eosinophil peroxidase into red fluorescent product) and their number was counted under a fluorescent microscope. The data indicate the number of cells per high-power field and represent mean ± SEM of four samples. b Mice were injected with 200 µg Alexa Fluor 647-labeled Nano-11 with 10 µg OVA and tissue samples were labeled as indicated. Confocal images were collected with a 60× objective lens and are representative of two independent experiments. Bar=10 µm