Fig. 3.

Immune recognition of each component within LEISH-F3+. C57BL/6 mice were injected a total of three times with 1 μg LEISH-F3+ protein, or a molar equivalence of the individual NH, SMT and ΔCPB proteins alone, formulated with GLA-SE. One month after the final immunization spleens were removed to prepare single cell suspensions (n = 3). In a, cells were incubated with antigen indicated in the box for 4 days then cytokine content in the culture supernatant determined by ELISA. In b, cells were subjected to flow cytometry to identify antigen-experienced CD4 T cells and the cytokine protection profile (various combinations of IFN-γ, IL-2, or TNF). Data are shown as mean and s.e.m, three mice per group. Data are representative of results obtained in two similar experiments