Figure 3.
PR-CARS analysis of the different characteristic morphological features visible in the demyelination process. (A) Total intensity images were taken from clinical score 1 (left) and 2 (right). Symbols (∗, #, ∗#) refer to different membrane locations. White square ROIs are used for statistical analysis in (B). Scale bars represent 5 μm. (B) (Left) Given here are S2 maps (superimposed on the intensity image) of characteristic features of myelin degradation in white ROIs of (A): “healthy” myelin, “swollen”, and “blebbed”; and products of degradation: “debris not filled (NF)” and “debris filled (F)”. (Middle) Given here are corresponding histograms of S2 values, taken within the dashed square ROIs in (B). (Right) Given here are combined images of S2 and φ2, showing S2 as a color scale and φ2 as the orientation of sticks for each measured pixel. (C) Given here is a schematic morphological interpretation of the myelin multilayer (enlarged) in positions in (A) corresponding to (∗) “healthy”, (∗#) “swollen”, and (#) “blebbed” myelin. The circular region represents the focal spot (not to scale). To see this figure in color, go online.