Fig. 2.

KK-Ay mice fasted overnight were sacrificed the next day after completing 10H2DA or vehicle administration. A: Relative pAMPK protein levels in skeletal muscles were determined by Western blotting (n=6). B: Relative mRNA expression levels of Pgc-1α in skeletal muscles (n=7–8) were quantified by qRT-PCR and normalized to the levels of Gapdh mRNA. C: Relative translocated GLUT4 protein levels in skeletal muscles were determined by Western blotting (n=6). Data are presented as the mean ± SEM. *P<0.05, **P<0.01 vs. vehicle.