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KK-Ay mice fasted overnight were sacrificed the next day after completing 10H2DA or vehicle administration. Relative mRNA expression levels of Pgc-1α in liver (n=7–8) (A), G6Pase in liver (n=7–8) (B) and Pck1 in liver (n=7–8) (C) were quantified by qRT-PCR and normalized to the levels of Gapdh mRNA. D: Relative pAMPK protein levels in liver (n=6) were determined by Western blotting. Data are presented as the mean ± SEM. *P<0.05, **P<0.01 vs. vehicle.
