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Calculated populations of duplexes formed in a 100 mM equimolar mixture of 3-mers in toluene (>50% dark blue, >30% royal blue, >20% pale blue, <20% pink). (a) Mixture of all eight trimer sequences. (b) Mixture that does not contain the alternating ADA and DAD sequences. Each duplex appears twice, and antiparallel sequence-complementary duplexes lie along the diagonal.
