Figure 3.

Free radical production rates by bc₁ complex in submitochondrial particles and intact mitochondria. (A) Model simulations of superoxide production by antimycin A-treated submitochondrial particles as a function of the Q-pool redox state using DQ kinetic constants as given in Table 1. (B) Model simulations of superoxide production by antimycin A-treated mitochondria as a function of the redox state of the Q pool using Q₁₀ kinetic constants as given in Table S1. (C) The semiforward and semireverse rates of superoxide production for the conditions given in (A). (D) The semiforward and semireverse rates of superoxide production for the conditions given in (B). To convert the superoxide production rate to H₂O₂ production, we assumed a stoichiometric relationship of two superoxide molecules per H₂O₂. Data in (A) are from Dröse and Brandt (57). Data in (B) are from Quinlan et al. (30). To see this figure in color, go online.