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. 2017 Sep 29;8:1701. doi: 10.3389/fpls.2017.01701

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Copyright © 2017 Aloisi, Cai, Faleri, Navazio, Serafini-Fracassini and Del Duca.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Co-treatment of pear pollen tubes with Spm and microtubule inhibitors. (A) DIC view of pollen tubes after co-treatment with Spm and taxol. (B) Detail of a single pollen tube after Spm+taxol treatment (DIC view). (C,D) DIC views of pollen tubes after co-treatment with Spm and oryzalin. Neither inhibitor affected the formation of the shovel shape. Images were captured after the incubation time indicated in each picture. Both analyses were performed at least until 1 h after supplementation of Spm and drugs. Bar in (A) 100 μm; bars in (B–D) 20 μm.