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Schematic overview of the proposed ascomycin biosynthesis pathways in S. hygroscopicus var. ascomyceticus. Dotted lines indicate more than one reaction step and the shaded boxes represent precursors of ascomycin biosynthesis. The overexpression targets in this study for improved production of ascomycin are indicated with green arrows, and the genes included in the ascomycin biosynthesis gene cluster are shown in red broken line boxes
