Fig. 2.

Timeline of the Mars500 experiment from the beginning (3rd of June, 2010) until the end (5th of November, 2011). The schematic drawing also indicates important steps and events during the confinement (above timeline) including the two off-nominal situations (critical situation simulations) and sampling dates from 18 sampling events. Red area/font denotes the stay of three marsonauts in the simulated Martian surface complex, whereas light blue area represents the timeframe where the facility was untenanted. One reference sampling was performed 6 months after confinement. Crosses represent samples that were used for PhyloChip analyses or NGS, respectively. Samples from each sampling were subjected to cultivation experiments. Red: medical module EU-100; green: habitable module EU-150; blue: utility module EU-250. Yellow stars indicate changing of NANO-filters and cleaning events of the primary filters on day 162 (11th of November, 2010) and 243 (2nd of February, 2011) of isolation