Fig. 7.

The BM stem/progenitor populations corresponding to HSC and MPP1–MPP4 (a) and each HPC fractions (b) were FACS analyzed. Representative flow cytometry scheme and quantification of the BM HSPC compartments were shown from Sall4 ^f/f, Sall4 ^f/f CreER^T2, and RosaCreER^T2mice 14 days after TAM treatment (n = 3 per group). PCR typing (4 mouse samples per group) and representative Western blotting assays for SALL4 deletion in TAM-treated Sall4 ^f/f; CreER^T2 mice are shown. c Representative flow cytometry scheme and quantification of the BM HSPC compartments from Sall4 ^f/fCre-, Sall4 ^f/+ /vavCre, and Sall4 ^f/f/vavCre mice (n = 4 per group, 2–3-weeks old). PCR typing showing Sall4 excision in indicated mouse BM cells. Error bars represent SD of at least three independent experiments. *p < 0.05 when compared to Sall4 ^f/f/CreERT; #p < 0.05 compared to Sall4 ^f/fCre-. LSK Lin-Sca1 + cKit+; GMP granulocyte-macrophage progenitor; CMP common myeloid progenitor; MEP megakaryocyte-erythroid progenitor