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. 2017 Sep 29;8:762. doi: 10.3389/fphys.2017.00762

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Copyright © 2017 Chu, Wang, Zheng, Lin, He, Liu and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Effect of high glucose or mannitol on MC apoptosis was measured by flow cytometry. Representative dot plots are shown, and percentage of cells positive for Annexin-V or positive for both Annexin-V and propidium iodide (PI) are represented as a histogram. Values are expressed as mean ± SEM (n = 3), ^*p < 0.05 vs. ctrl; ^**p < 0.01 vs. ctrl; ++p < 0.01 vs. 236 mM mannitol. (B) Apoptotic rate of high-glucose-treated MCs transfected with HMGB1 siRNA or incubated with rHMGB1 was tested by flow cytometry. NS means no significant difference.