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Virtual 2D-PAGE comparing HCPs identified in the null cell immunogen without ELISA coverage (gray) with HCPs that have unambiguous immunoreactivity with the anti-HCP reagent (red) as assessed by unique affinity-purification MS identification relative to negative controls. Proteins recognized by the ELISA reagent span the molecular weight and pI range. Spot size is scaled relative to protein abundance in the immunogen according to Mascot emPAI statistic.
