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. 2017 Jul 14;9(7):1065–1075. doi: 10.1080/19420862.2017.1349586

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© 2017 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted

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Figure 6. — Virtual 2D-DIGE comparison of downstream impurities identified in the Protein A eluate of mAb A (red) and HCPs with ELISA coverage identified uniquely by anti-HCP affinity purification (gray). Protein A impurities with a concentric gray dot have confirmed ELISA immunoreactivity. Protein A impurity spot size is scaled relative to protein abundance, while fixed spot size is used for HCPs identified by anti-HCP affinity purification.