Table 1.

Surface and molecular attributes of mAbA, mAbB and mAbC. Molecular weights of mAbA, mAbB and mAbC were determined by analyzing the monomer peak eluted from a size exclusion chromatography column using a miniDawn TREOS MALS detector. Partial molar volume of the antibodies was determined by performing a densimetry assay as a function of antibody concentration in 25 mM Na-acetate buffer at pH 5.5 at 20°C. Total point charges were determined by summing the predicted point charges of solvent accessible residues at pH 5.5 using a homology model of mAbA, mAbB and mAbC. The surface aggregation propensity (SAP) score which is a measure of the surface hydrophobicity of mAb molecules was calculated using molecular dynamics simulations.

	Molecular weight	Partial molar volume ($\overline{\text{V}}$2)	Charge			Hydrophobicity
Antibody	(kDa)	(L/mol)	Positive	Negative	Net	SAP score
mAbA	150.3 ± 0.4	104.6 ± 0.003	151	113	+38	577 ± 8
mAbB	143.8 ± 0.1	103.6 ± 0.02	157	116	+41	581 ± 4
mAbC	144.9 ± 0.1	103.7 ± 0.01	145	115	+30	677 ± 5