Table 1.

Binding constants of mAb1 and Fabs thereof as determined by surface plasmon resonance.

Sample	ka (1/Ms)	kd (1/s)	KD (nM)
IgG1	1.61E + 05	2.96E - 05	0.2
Recombinant Fab	9.03E + 04	7.37E - 05	0.8
Purified Fab following an in-solution papain digest	7.95E + 04	8.02E - 05	1.0
In-solution papain digest (1 h) added antipain	2.23E + 04	4.24E - 05	1.9
In-solution papain digest (3.5 h) added antipain	n.d.	n.d.	n.d.
GingisKHAN™ digest	5.18E + 04	5.83E - 05	1.1

Abbreviations: k_a, association rate constant; k_d, dissociation rate constant; K_D, binding constant; mAb1, a humanized IgG1 binding dimeric Vascular Endothelial Growth Factor A; n.d., not detectable