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. 2017 Aug 17;9(7):1052–1064. doi: 10.1080/19420862.2017.1364824

Figure 3.

Figure 3.

TaqMan® Copy Number assay of stable cell lines. Stable cell lines were generated using either separate transfection of each of the pQL13-based Env/V3 chimeras or a mixture of all 5 Env/V3 chimeras for transfection (HEK293 cell library). Relative copy numbers of integrated pQL13 plasmids were explored using a TaqMan Copy Number Assay on 4 individual samples of genomic DNA of each cell line and probing for eGFP in relation to the human telomerase reverse transcriptase (TERT) genes, resulting in an integration rate of 1 for each human cell line (differences between the 6 stable cell lines statistically n.s., p > 0.05).