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Correlated expression of V3 envelope variants and eGFP. 3 × 105 cells of each HEK293 stable cell line were induced with doxycycline (1 µg/ml) 24 h before flow cytometry analysis. The cells were harvested and stained with 5F3 and goat anti-human-APC as a secondary antibody. Measurements were performed in duplicates. 2.5 × 104 cells were recorded per sample and the correlation between APC and GFP signal intensities was analyzed for each stable cell line (3000 events shown).
