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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: IEEE J Sel Top Quantum Electron. 2016 Dec 14;23(2):6900610. doi: 10.1109/JSTQE.2016.2639824

Figure 4.

Figure 4

A) Normalized intensity of center pixel for three sizes of polystyrene nanospheres bound to a 30nm oxide on silicon substrate. Significant changes in appearance and defocus behavior are observed due to variation in z-axis position of the radiating dipole with respect to the reference field generated by the reflective surface. B–D) Line profiles of the observed appearance for the three nanospheres simulated for three focal plane offsets (−.5, 0, and +.5 μm) with respect to the oxide surface. Simulations were generated for an .9NA in-water imaging system. Circles in plots B–D represent pixel sampling locations for a 2 μm pixel pitch with 40× magnification.