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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: IEEE J Sel Top Quantum Electron. 2016 Dec 14;23(2):6900610. doi: 10.1109/JSTQE.2016.2639824

Figure 5.

Figure 5

A) Shift in the normalized intensity of center pixels for two sizes of polystyrene spheres (100nm and 150nm) due to the presence of a 10 nm biofilm of capture probes. B–C) Line profiles of the observed appearance for the three nanospheres simulated for two focal plane offsets (−.5 and +.5 μm) with respect to the oxide surface. Simulations were generated for an .9NA in-water imaging system. Circles in plots B and C represent pixel sampling locations for a 2 μm pixel pitch with 40× magnification.