FIG. 2.

HPLC chromatograms of C. sativa ethanolic extracts. Chromatogram of fresh Cannabis extract (C2F; 0.1 mg/mL) and baked (i.e., fresh flowers that were baked at 150°C for 3 h) Cannabis extract (C2B; 0.1 mg/mL) obtained from isocratic elution with a mixture of 15% water containing 0.1% acetic acid (solvent A) and 85% MeOH (solvent B) for a total run time of 35 min at 220 nm. The samples were injected at a concentration of 0.58 mg/mL in a volume of 20 μL for C2B and 0.33 mg/mL in a volume of 20 μL for C2F.