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HPLC profile of C2F, F7, and THCA. Chromatograms of THCA standard at 40 ppm (marked in blue), whole C. sativa extract at 0.1 mg/mL (marked in green), and F7 at 0.04 mg/mL (marked in red). All samples were injected in a volume of 20 μL and were obtained from isocratic elution with a mixture of 15% water containing 0.1% acetic acid (solvent A) and 85% MeOH (solvent B) for a total run time of 40 min at 220 nm. THCA, Δ9-tetrahydrocannabinolic acid.
