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. 2017 Oct;58(12):4959–4975. doi: 10.1167/iovs.17-21531

Figure 3.

Figure 3

Loss of SDC1 leads to reduced mRNA expression for SDC2 and SDC4 and increased expression of SDC3 mRNA and protein within SDC1-null ICNs. (A) Quantitative PCR was performed using primers for SDC1, SDC2, SDC3, and SDC4 on RNA extracts obtained by limbal to limbal debridement from unwounded 7- to 9-week-old WT and SDC1-null corneas. Data show that RNA for SDC2 and SDC4 are significantly reduced in SDC1-null mice, whereas SDC3 is increased. (B) Because SDC3 is expressed in the CNS and PNS, we next stained corneas with antibodies against SDC3 (green) and βIII tubulin (red). In WT and SDC1-null corneas, SDC3 is localized on short segments of the stromal nerves as they exit the epithelium and become ICNs (*). In SDC1-null corneas, numerous ICNs show faint but detectible expression of SDC3 compared with WT corneas. Scale bar: 10 μm.