Figure 1.

Western blot analysis of STAT3 phosphorylation by T/C28a2 and C28/I2 chondrocytes.

Notes: T/C28a2 (A) or C28/I2 (B) chondrocytes were held at 25°C (no incubation) or incubated at 37°C for 30 min with “no additions,” “no additions” plus DMSO, rhIL-6 (50 ng/mL), sIL-6Rα (30 ng/mL), Janex-1 (100 μM), or various combinations thereof. Western blots were produced with either an anti-U-STAT3 (STAT3) antibody or an anti-p-STAT3 antibody.

Abbreviations: DMSO, dimethyl sulfoxide; sIL-6Rα, soluble IL-6 receptor α; STAT, signal transducers and activators of transcription.