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. 2017 Sep 27;12:7153–7163. doi: 10.2147/IJN.S140557

Figure 4.

Figure 4

Effects of LNC, AcE and AcE-LNC on chemical mediator secretion by melanoma cells cocultured with murine neutrophils. B16F10 cells (1×104 cells/well) were incubated with vehicle (R10), LNC (9×109 particles/mL), AcE (30 µM) or AcE-LNC (30 µM of AcE; 9×109 particles/mL) for 24 h, washed and then co-incubated or not with murine neutrophils (5×105 cells/transwell) for 18 h.

Notes: Supernatant of cell cultures was used to quantify VEGF-A (A), IL-10 (B), Arginase-1 (C) and MMP-9 (D) by ELISA. Significant differences are aP<0.05 vs respective vehicle; bP<0.05 vs respective neutrophils; cP<0.05 vs respective AcE; and dP<0.05 vs respective LNC. AcE-LNC, LNC loaded with AcE.

Abbreviations: AcE, acetyleugenol; ELISA, enzyme-linked immunosorbent assay; IL-10, interleukin-10; LNC, lipid-core nanocapsule; Neut, neutrophils; MMP-9, matrix metalloproteinase-9; VEGF, vascular endothelial growth factor.