Figure 5.

Effects of LNC, AcE and AcE-LNC on leukocyte–endothelial interactions in vivo.

Notes: C57Bl/6 mice were anesthetized, and cremaster muscle was exposed to intravital microscopy study. (A) Number of rolling leukocytes was evaluated before (time 0) and 10, 30, 60 and 120 min after i.v. administration of vehicle (saline), LNC (0.9±0.5×10¹²), AcE (20 mg/kg) or AcE-LNC (20 mg/kg; 0.72±0.4×10*12, as LNC). (B) Representative images of microcirculation before and 120 min after i.v. administration of vehicle (saline), LNC (0.9±0.5×10¹²), AcE (20 mg/kg) or AcE-LNC (20 mg/kg; 0.72±0.4×10*12, as LNC). Arrows indicate rolling leukocytes. Results are expressed as mean ± SEM of 5–6 animals in each group. Significant differences are ^aP<0.05 vs respective vehicle; ^bP<0.05 vs respective LNC and ^cP<0.05 vs respective AcE. AcE-LNC, LNC loaded with AcE.

Abbreviations: AcE, acetyleugenol; i.v., intravenous; LNC, lipid-core nanocapsule; SEM, standard error of the mean.