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. 2017 Sep 19;40(5):1495–1503. doi: 10.3892/ijmm.2017.3143

Figure 5.

Figure 5

Mitogen-activated protein kinase (MAPK)/p38 signaling pathway inhibitor pretreatment repressed cytoskeleton rearrangement and production of interleukin (IL)-6 and interferon (IFN)-β. (A–C) RAW264.7 cells were primed for 30 min with PD98059, SB203580 and SP600125, and then stimulated with 100 ng/ml lipopolysaccharide (LPS). (A) The levels of non-phosphorylated and phosphorylated extracellular signal-regulated kinase (ERK), p38 and c-Jun N-terminal kinase (JNK) in the lysates were detected by immunoblotting. (B) IL-6 and IFN-β expression was measured by quantitative polymerase chain reaction (**P<0.05, ***P<0.001 and ##P<0.01). (C) Cell morphology was observed under a light microscope (original magnification, ×100). RAW264.7 cells were primed for 30 min with vincristine (VCR) and stimulated with 100 ng/ml LPS for 30 min. Subsequently, p38 and p65 phosphorylation were detected by immunoblotting (D). Similar observations were obtained from three independent experiments. DMSO, dimethyl sulfoxide.