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. 2017 Sep 13;40(5):1415–1425. doi: 10.3892/ijmm.2017.3131

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Copyright: © Ma et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Changes in the expression of angiogenin-1 (Ang-1) and the phosphorylation of tyrosine kinase receptor-2 (p-Tie-2). (A) Gene expression levels of Ang-1 were assessed by RT-qPCR. (B) Representative western blots showing the protein expression of Ang-1 in the different groups. Representative western blots showing Tie2 tyrosine phosphorylation levels in the different groups. (C) Statistical analysis of Ang-1 protein expression levels. (D) Immunohistochemical staining of Ang-1 protein on day 7 after the surgery. The Ang-1 protein signals were mainly present in pericyte-like perivascular mural cells (red arrows). (E) Quantify analysis the expression of p-Tie-2 in the different groups. (F) Immunohistochemical staining of Tie-2 in the different groups on day 7. Tie-2 protein signals were mainly present in endothelial cells (red arrows). Scale bar, 50 µm. All data are expressed as the means ± SD (^*P<0.05 and ^**P<0.01).