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. 2017 Sep 14;40(5):1466–1476. doi: 10.3892/ijmm.2017.3135

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Copyright: © Yang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Double-label immunofluorescence assay in RLE-6TN cells. Surfactant protein C (SPC), red fluorescence; α-smooth muscle actin (α-SMA), green fluorescence; nucleus, blue fluorescence. In both the control and the Runx3 groups, SPC was expressed in the cytoplasm. In the transforming growth factor-β1 (TGF-β1) group and the siRunx3 group, α-SMA expression was detectable in the cytoplasm. In the Runx3 + TGF-β1 group, a large amount of SPC expression and a trace amount of α-SMA expression were found.