Figure 4.

miR-135a directly targets transient receptor potential channel 1 (TRPC1) 3′ untranslated region (UTR). (A) Schematic diagram of TRPC1 3′UTR reporter construct. (B) Sequence alignment between miR-135a and mouse TRPC1 3′UTR, including wild-type (wt) and mutant (mut). (C) For dual Luciferase reporter assays, 293T cells were transfected with pCDNA3.1-Luciferase TRPC1-3′UTR reporter and miR-135a mimics. (D) Reverse transcription-quantitative polymerase chain reaction analysis was used to determine the effect of miR-135a on TRPC1 mRNA expression. (E) The effect of miR-135a on TRPC1 protein expression was determined by western blot analysis. (F) Quantitative analysis of the results of (E). ^*P<0.05 and ^**P<0.01 indicate statistically significant and highly statistically significant differences, respectively, compared with the controls.