Table I.
Name | Primer sequences |
---|---|
TRPC1 3′UTR | |
Sense | 5′-TGTATTTGCATACTTGCAA-3′ |
Antisense | 5′-GAGGGAACACTTAAACCTG-3′ |
TRPC1 3′UTR (mutant) | |
Sense | 5′-TGTATTTGCATACTTGCAA-3′ |
5′-CAATTACACATACGGCTTTCTT-3′ | |
Antisense | 5′-AAGAAAGCCGTATGTGTAATTG-3′ |
5′-GAGGGAACACTTAAACCTG-3′ | |
TRPC1 CDS | |
Sense | 5′-ATGGGGGCCCCGCCTCCGTCT-3′ |
Antisense | 5′-TTAATTTCTTGGATAAAACATAG-3′ |
PCR, polymerase chain reaction; TRPC1, transient receptor potential channel 1; UTR, untranslated region; CDS, coding sequence.