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. 2017 Sep 25;40(5):1511–1519. doi: 10.3892/ijmm.2017.3152

Table I.

Sequences of the PCR primers for construction.

Name Primer sequences
TRPC1 3′UTR
 Sense 5′-TGTATTTGCATACTTGCAA-3′
 Antisense 5′-GAGGGAACACTTAAACCTG-3′
TRPC1 3′UTR (mutant)
 Sense 5′-TGTATTTGCATACTTGCAA-3′
5′-CAATTACACATACGGCTTTCTT-3′
 Antisense 5′-AAGAAAGCCGTATGTGTAATTG-3′
5′-GAGGGAACACTTAAACCTG-3′
TRPC1 CDS
 Sense 5′-ATGGGGGCCCCGCCTCCGTCT-3′
 Antisense 5′-TTAATTTCTTGGATAAAACATAG-3′

PCR, polymerase chain reaction; TRPC1, transient receptor potential channel 1; UTR, untranslated region; CDS, coding sequence.