Skip to main content
. 2017 Sep 19;40(5):1477–1485. doi: 10.3892/ijmm.2017.3140

Figure 2.

Figure 2

TM-induced ER stress induced FGF21 expression and increased circulating FGF21 expression levels in H9c2 cells. (A) H9c2 cells were treated with various concentrations (1, 5, 10, 20 and 50 µM) of TM for 24 h. The expression levels of FGF21, FGFR1, p-FGFR1 and β-Klotho in H9c2 cells in response to TM-induced ER stress were evaluated by western blot analysis. β-actin served as a loading control. (B) Secreted FGF21 levels in culture medium following ER stress stimulation were measured by ELISA assay. *P<0.05 or **P<0.01 vs. control. TM, tunicamycin; ER, endoplasmic reticulum; FGF21, fibroblast growth factor 21; FGFR1, fibroblast growth factor receptor 1; p, phosphorylated; c, control.