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. 2017 Sep 19;40(5):1477–1485. doi: 10.3892/ijmm.2017.3140

Figure 3.

Figure 3

Overexpressed FGF21 protected H9c2 cells from ER stress-induced apoptosis. (A) The recombinant plasmid pcDNA4-FGF21 was constructed by pcDNA4/To/myc-his (5,151 bp) and full-length rat FGF21 (627 bp), confirmed by agarose gel electrophoresis and gene sequencing. (B) Western blot analysis demonstrating the overexpression of myc-labelled FGF21 protein from the transfected pcDNA4-FGF21 plasmid in H9c2 cells using a c-myc tag and FGF21 antibodies. (C) H9c2 cells were transfected with pcDNA4-FGF21 plasmid for 48 h, and exposed to TM (10 µM) treatment for a further 24 h, the cell viability was detected by Cell Counting Kit-8 assay. (D) Fluorescence microscopy of TUNEL staining of H9c2 cells. Cell nucleus was stained with DAPI (blue). Scale bar, 50 µm. The percentage of apoptotic cells in each group was calculated by counting the condensed nuclei. **P<0.01 vs. control, &P<0.05 vs. TM-treated cells, #P<0.05 vs. pcDNA4 + TM group. FGF21, fibroblast growth factor 21; ER, endoplasmic reticulum; TM, tunicamycin; TUNEL, terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling; DAPI, 4′,6-diamidino-2-phenylindole.