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. 2017 Sep 7;40(5):1307–1314. doi: 10.3892/ijmm.2017.3124

Figure 3.

Figure 3

miR-132 inhibits tumor suppressor candidate 3 (TUSC3) protein expression in U87MG cells. (A) Schematic of predicted miR-132-binding sites in the 3′UTR of TUSC3 mRNA by miRanda. (B) Real-time PCR for miR-132 in U87MG cells transfected with pre-miR-132 or control miR. U6 was used as a loading control; n=3 experiments. (C) Western blot analysis for TUSC3 in U87MG cells transfected with pre-miR-132 and control miR (mock). β-actin was used as a loading control; n=3 experiments. (D) Immunofluorescence assay for TUSC3 in U87MG cells transfected with pre-miR-132 or control miR (mock); n=3 experiments. (E) RT-PCR for TUSC3 in U87MG cells transfected with pre-miR-132 or control miR (mock). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control; n=3 experiments. (F) Real-time PCR for TUSC3 in U87MG cells transfected with pre-miR-132 or control miR (mock). GAPDH was used a loading control; n=3 experiments.