Figure 5.

miR-132 induces the formation of glioblastoma-initiating cell (GICs) phenotype in U87MG cells. (A) Sphere growth in U87MG cells transfected with pre-miR-132 or control miR (mock). The data are reported as the number of spheres formed/1,000 seeded cells; n=3 experiments. (B) Western blot analysis for MET, CD133 and ZFX in U87MG cells transfected with pre-miR-132 or control miR (mock). β-actin was used as a loading control; n=3 experiments. (C) Clonogenic agar assay for U87MG cells transfected with pre-miR-132 or control miR (mock); n=3 experiments.