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. 2017 Sep 6;40(5):1537–1548. doi: 10.3892/ijmm.2017.3121

Figure 6.

Figure 6

Levels of autophagy-related protein Beclin-1 after RSLT. (A) Levels of Beclin-1 protein as assessed immunohistochemically on POD 0, POD 1, POD 5, POD 7, POD 10 and POD 14. Only a small amount of Beclin-1 protein was present in the cytoplasm of hepatocytes around the central vein on POD 0, which was similar in all three groups. Beclin-1 could also be observed in the biliary epithelia on POD 1. The protein expression increased after POD 5, extending to the cytoplasm of most hepatocytes and biliary endothelia. The overall protein level in the HO-1/BM-MSCs-treated group was the highest and was much higher than that in the normal saline (NS)-treated group and the BM-MSCs-treated group. (B and C) Levels of the Beclin-1 protein as demonstrated by western blotting on POD 0, POD 1, POD 3, POD 5, POD 7, POD 10 and POD 14. The protein abundance in the BM-MSCs-treated group was significantly higher than that in the NS-treated group at each time-point, except on POD 5, POD 7 and POD 14. The protein abundance in the HO-1/BM-MSCs-treated group was significantly higher than that in the NS-treated group and BM-MSCs-treated group at each time-point, except on POD 0. *P<0.05 as indicated. RSLT, reduced-size liver transplantation; POD, post-operative day; HO-1/BM-MSCs, HO-1 transduced BM-MSCs; BM-MSCs, bone marrow mesenchymal stem cells.