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. 2017 Sep 20;40(5):1405–1414. doi: 10.3892/ijmm.2017.3145

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of autophagy in LX-2 cells incubated with caffeine. (A) The viability of LX-2 cells transfected with Atg7-siRNAs or the cells transfected with negative siRNAs after 20 mM caffeine treatment at the indicated times was detected by CCK-8 assay. (B) The viability of LX-2 cells treated with 3-MA (5 mM), caffeine (20 mM) and caffeine (20 mM) together with 3-MA (5 mM) was detected by CCK-8 assay. (C) The percentage of apoptosis of LX-2 cells transfected with Atg7-siRNAs or the cells transfected with negative siRNAs after 20 mM caffeine treatment for 48 h was detected by flow cytometry. Bars represent the means ± SD of triplicate experiments. ^**P<0.01. (D) The percentage of apoptotic LX-2 cells treated with 3-MA (5 mM), caffeine (20 mM) and caffeine (20 mM) together with 3-MA (5 mM) was detected by flow cytometry. Bars represent the means ± SD of triplicate experiments. ^*P<0.05. (E) Western blotting and densitometric analysis show the expression of p62, α-SMA and LC3II in LX-2 cells transfected with Atg7-siRNAs or the cells transfected with negative siRNAs after treatment with 0/20 mM caffeine for 48 h. Bars represent the means ±SD of three independent experiments. ^**P<0.01. (F) Western blotting and densitometric analysis show the expression of p62, α-SMA and LC3II in LX-2 cells treated with 3-MA (5 mM), caffeine (20 mM), caffeine (20 mM) together with 3-MA (5 mM) and none. Bars represent the means ± SD of three independent experiments. ^**P<0.01.