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. 2017 Sep 19;40(5):1486–1494. doi: 10.3892/ijmm.2017.3142

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Copyright: © Heo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Time course images of osteogenic differentiation and expression of osteogenesis-related markers. Top panels: images of BM-MSC1 induced by osteogenic medium, and RT-PCR analysis of osteogenesis-associated markers in BM-MSC1. Second row: images of BM-MSC2 differentiated by osteogenic medium, and RT-PCR analysis of osteogenesis-related markers in BM-MSC2. Third row: images of CB-MSC1 induced by osteogenic medium, and RT-PCR analysis of osteogenesis-associated markers in CB-MSC1. Lower panels: images of CB-MSC2 induced by osteogenic medium, and RT-PCR analysis of osteogenesis-related markers in CB-MSC2. Osteogenesis was evaluated by Von Kossa staining (scale bar, 100 µm; magnification, ×200).