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Effect of tanshinone IIA on osteogenesis. (A) Mesenchymal stem cells (MSCs) treated with tanshinone IIA were photographed after 3 days (scale bar, 200 µm; magnification, ×100). (B) Cell viability was determined by trypan blue staining. The cell viability of the tanshinone IIA-treated MSCs was >90%. (C) Induction of osteogenesis-associated genes by tanshinone IIA treatment. Treatment only with 6 µM tanshinone IIA induced BMP2, distal-less homeobox 5 (DLX5) and osterix genes.
