Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 19;40(5):1486–1494. doi: 10.3892/ijmm.2017.3142

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Heo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Changes in mesenchymal stem cells (MSCs) following tanshinone IIA treatment. (A) RT-PCR analysis of osteogenesis-, chondrogenesis- and adipogenesis-associated genes in the control and tanshinone IIA-induced MSCs. Increased osteogenic differentiation of MSCs by tanshinone IIA. (B) Induction of distal-less homeobox 5 (DLX5) expression enhanced osteogenesis of DLX5 not-expressing MSCs. The differentiation capacity of the cells was analyzed after induction. (a) Osteogenesis assessed by Von Kossa staining (scale bar, 50 µm). (b) Chondrogenesis assessed by safranin O staining (scale bar, 100 µm). (c) Adipogenesis assessed by Oil Red O staining (scale bar, 50 µm). (C) Calcium concentrations were measured in triplicate using a Calcium LiquiColor test. Relative mRNA expression levels of differentiation-associated markers in the control and DLX5-induced MSCs after (D) osteogenic induction, (E) chondrogenic induction, and (F) adipogenic induction. ^*P<0.05 and ^**P<0.01.