Table I.
Antibodies used for immunohistochemical, immunocytochemical and western blot analysis.
| Primary antibody | Supplier (location) | Secondary antibody/fluorochrome | Supplier (location) |
|---|---|---|---|
|
Nestin/M immunohistochemistry MAB5326; 1:200 |
Merck KGaA (Darmstadt, Germany) | Goat anti-mouse/TRITC 115-025-044; 1:30 | Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA, USA) |
|
Nestin/P HPA007007; 1:200 Oct-4/P P0056; 1:200 |
Sigma-Aldrich (Merck KGaA; Darmstadt, Germany) | Swine anti-rabbit/FITC F0205; 1:30 | Dako (Agilent Technologies, Inc., Santa Clara, CA, USA) |
|
CD45/M C7556; 1:50 Vimentin/M M0725; 1:50 |
Dako (Agilent Technologies, Inc., Santa Clara, CA, USA) | Goat anti-mouse/TRITC T5393; 1:30 | Sigma-Aldrich (Merck KGaA; Darmstadt, Germany) |
|
Smooth muscle actin/M M0851; 1:50 |
|||
|
Nanog/M AF1997; 1:15 |
R&D Systems, Inc. (Minneapolis, MN, USA) | Donkey anti-goat/TRITC Sc-2094; 1:200 | Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) |
| Melan A/M MA5-14168; 1:100 HMB-45 (anti-melanosome)/M 081050 ready to use antibody |
Invitrogen (Thermo Fisher Scientific, Inc., Waltham, MA, USA) | Goat anti-mouse/TRITC 115-025-044; 1:30 | Sigma-Aldrich (Merck KGaA; Darmstadt, Germany) |
|
Wide spectrum cytokeratin/P Ab9377; 1:100 |
Abcam (Cambridge, UK) | Swine anti-rabbit/FITC F0205; 1:30 | Dako (Agilent Technologies, Inc., Santa Clara, CA, USA) |
|
α-Tubulin (loading control) T9026; 1:500 |
Sigma-Aldrich (Merck KGaA; Darmstadt, Germany) | Goat anti-mouse/horseradish peroxidase Sc-516102; 1:2,000 | Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) |
TRITC, tetramethylrhodamine; FITC, fluorescein isothiocyanate.