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. 2017 Sep 6;40(5):1435–1443. doi: 10.3892/ijmm.2017.3120

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Copyright: © Guo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of miR-7-5p, TFF3 and LY294002 on the migration of LS174T cells. (A) The crystal violet staining results of LS174T cell migration through the Transwell chamber (magnification, ×200): (a) LS174T cells transfected with miR-7-5p mimic NC; (b) LS174T cells transfected with miR-7-5p mimics; (c) LS174T cells transfected with miR-7-5p mimics and TFF3 plasmid; (d) LS174T cells transfected with miR-7-5p mimic NC and LY294002; (e) LS174T cells transfected with miR-7-5p mimics and LY294002; (f) LS174T cells transfected with miR-7-5p mimics, TFF3 plasmid and LY294002. (B) Histogram of the number of LS174T cells migrating through the Transwell chamber. The assays were performed in triplicate. All values are presented as the means ± standard deviation of three replicates. miR. ^*P<0.05. miR, microRNA; TFF3, trefoil factor 3; NC, negative control.