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. 2017 Sep 6;40(5):1435–1443. doi: 10.3892/ijmm.2017.3120

Figure 4.

Figure 4

(A) Western blot detection of TFF3, PI3K, Akt and p-Akt expression levels in cells with overexpressed or downregulated miR-7-5p levels. GAPDH served as the loading control. (B) Quantitative analysis of TFF3, PI3K, Akt and p-Akt protein levels normalized to GAPDH levels. The assays were performed in triplicate. All values are presented as the means ± standard deviation of three replicates. *P<0.05 compared with NC, miR-7-5p mimic NC and miR-7-5p inhibitor NC groups. TFF3, trefoil factor 3; PI3K, phosphoinositide 3-kinase; p, phosphorylated; miR, microRNA; GAPDH, glyceraldehydes-3-phosphate dehydrogenase.